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KMID : 0380020150300050230
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 5 p.230 ~ p.238
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Recent Advances in MALDI-MS Based Quantitative Targeted Glycan Analysis
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Kim Kyoung-Jin
Kim Yoon-Woo
Hwang Cheol-Hwan
Park Han-Kyu
Jeong Jae-Hyun
Kim Yun-Gon
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Abstract
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Abnormal glycosylation can significantly affect the intrinsic functions (i.e., stability and solubility) of proteins and the extrinsic protein interactions with other biomolecules. For example, recombinant glycoprotein therapeutics needs proper glycosylation for optimal drug efficacy. Therefore, there has been a strong demand for rapid, sensitive and high-throughput glycomics tools for real-time monitoring and fast validation of the biotherapeutics glycosylation. Although liquid chromatography tandem mass spectrometry (LC-MS/MS) is one of the most powerful tools for the characterization of glycan structures, it is generally time consuming and requires highly skilled personnel to collect the data and analyze the results. Recently, as an alternative method, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS), which is a fast, robust and easy-to-use instrumentation, has been used for quantitative glycomics with various chemical derivatization techniques. In this review, we highlight the recent advances in MALDI-MS based quantitative glycan analysis according to the chemical derivatization strategies. Moreover, we address the application of MALDIMS for high-throughput glycan analysis in many fields of clinical and biochemical engineering.
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KEYWORD
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MALDI-MS, Recombinant glycoprotein drug, Glycan, Quantitative analysis, Chemical derivatization, Highthroughput analysis
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